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Registro Completo |
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
24/09/2013 |
Data da última atualização: |
05/04/2024 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
LACERDA, A. E. B. de; BORGES, M. V. K. |
Afiliação: |
ANDRE EDUARDO BISCAIA DE LACERDA, CPATU; MARCELUS VINICIUS K. BORGES, ASSOCIAÇÃO CAIGUARA DE PESQUISAS. |
Título: |
Caracterização fito-ecológico e ambiental das áreas aluvionares na região. |
Ano de publicação: |
2003 |
Fonte/Imprenta: |
In: CONGRESSO DE ECOLOGIA DO BRASIL, 6., 2003, Fortaleza. Anais de trabalhos completos. Fortaleza: Editora da Universidade Federal do Ceará, 2003. |
Páginas: |
p. 608-610. |
Idioma: |
Português |
Palavras-Chave: |
Floresta de galeria. |
Thesagro: |
Botânica; Ecologia; Taxonomia vegetal; Varzea. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00646nam a2200181 a 4500 001 1966929 005 2024-04-05 008 2003 bl uuuu u00u1 u #d 100 1 $aLACERDA, A. E. B. de 245 $aCaracterização fito-ecológico e ambiental das áreas aluvionares na região.$h[electronic resource] 260 $aIn: CONGRESSO DE ECOLOGIA DO BRASIL, 6., 2003, Fortaleza. Anais de trabalhos completos. Fortaleza: Editora da Universidade Federal do Ceará$c2003 300 $ap. 608-610. 650 $aBotânica 650 $aEcologia 650 $aTaxonomia vegetal 650 $aVarzea 653 $aFloresta de galeria 700 1 $aBORGES, M. V. K.
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Embrapa Amazônia Oriental (CPATU) |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
Data corrente: |
07/02/2019 |
Data da última atualização: |
07/01/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
PEREIRA, C. M.; CARVALHO, A. C. de; SILVA, F. R. da; MELENDEZ, M. E.; LESSA, R. C.; ANDRADE, V. C. C.; KOWALSKI, L. P.; VETTORE, A. L.; CARVALHO, A. L. |
Afiliação: |
CLÁUDIA MARIA PEREIRA, A. C. Camargo Cancer Hospital, Ludwig Institute for Cancer Research, São Paulo; ANA CAROLINA DE CARVALHO, Barretos Cancer Hospital, Unifesp; FELIPE RODRIGUES DA SILVA, CNPTIA; MATIAS ELISEO MELENDEZ, Barretos Cancer Hospital; ROBERTA CARDIM LESSA, A. C. Camargo Cancer Hospital, Ludwig Institute for Cancer Research; VALÉRIA CRISTINA C. ANDRADE, Unifesp; LUIZ PAULO KOWALSKI, A. C. Camargo Cancer Hospital; ANDRÉ L. VETTORE, Ludwig Institute for Cancer Research, Unifesp; ANDRÉ LOPES CARVALHO, A. C. Camargo Cancer Hospital, Barretos Cancer Hospital, Barretos. |
Título: |
In vitro and in silico validation of CA3 and FHL1 downregulation in oral cancer. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
BMC Cancer, v. 18, p. 1-10, 2018. |
DOI: |
https://doi.org/10.1186/s12885-018-4077-3 |
Idioma: |
Inglês |
Notas: |
Article number: 193. |
Conteúdo: |
Abstract. Background: Aberrant methylation is a frequent event in oral cancer. Methods: In order to better characterize these alterations, a search for genes downregulated by aberrant methylation in oral squamous cell carcinoma (OSCC) was conducted through the mining of ORESTES dataset. Findings were further validated in OSCC cell lines and patients? samples and confirmed using TCGA data. Differentially expressed genes were identified in ORESTES libraries and validated in vitro using RT-PCR in HNSCC cell-lines and OSCC tumor samples. Further confirmation of these results was performed using mRNA expression and methylation data from The Cancer Genome Atlas (TCGA) data. Results: From the set of genes selected for validation, CA3 and FHL1 were downregulated in 60% (12/20) and 75% (15/20) of OSCC samples, respectively, and in HNSCC cell lines. The treatment of cell lines JHU-13 and FaDu with the demethylating agent 5'-aza-dC was efficient in restoring CA3 and FHL1 expression. TCGA expression and methylation data on OSCC confirms the downregulation of these genes in OSCC samples and also suggests that expression of CA3 and FHL1 is probably regulated by methylation. The downregulation of CA3 and FHL1 observed in silico was validated in HNSCC cell lines and OSCC samples, showing the feasibility of integrating different datasets to select differentially expressed genes in silico. Conclusions: These results showed that the downregulation of CA3 and FHL1 data observed in the ORESTES libraries was validated in HNSCC cell lines and OSCC samples and in a large cohort of samples from the TCGA database. Moreover, it suggests that expression of CA3 and FHL1 could probably be regulated by methylation having an important role the oral carcinogenesis. MenosAbstract. Background: Aberrant methylation is a frequent event in oral cancer. Methods: In order to better characterize these alterations, a search for genes downregulated by aberrant methylation in oral squamous cell carcinoma (OSCC) was conducted through the mining of ORESTES dataset. Findings were further validated in OSCC cell lines and patients? samples and confirmed using TCGA data. Differentially expressed genes were identified in ORESTES libraries and validated in vitro using RT-PCR in HNSCC cell-lines and OSCC tumor samples. Further confirmation of these results was performed using mRNA expression and methylation data from The Cancer Genome Atlas (TCGA) data. Results: From the set of genes selected for validation, CA3 and FHL1 were downregulated in 60% (12/20) and 75% (15/20) of OSCC samples, respectively, and in HNSCC cell lines. The treatment of cell lines JHU-13 and FaDu with the demethylating agent 5'-aza-dC was efficient in restoring CA3 and FHL1 expression. TCGA expression and methylation data on OSCC confirms the downregulation of these genes in OSCC samples and also suggests that expression of CA3 and FHL1 is probably regulated by methylation. The downregulation of CA3 and FHL1 observed in silico was validated in HNSCC cell lines and OSCC samples, showing the feasibility of integrating different datasets to select differentially expressed genes in silico. Conclusions: These results showed that the downregulation of CA3 and FHL1 data observed in the ORESTES l... Mostrar Tudo |
Palavras-Chave: |
Carcinogênese; Expressão gênica; Open reading expressed sequence tags; Oral squamous cell carcinoma; ORESTES; Validação in silico; Validação in vitro. |
Thesagro: |
Metilação. |
Thesaurus NAL: |
Gene expression; Methylation. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/192300/1/AP-InVitro-Felipe.pdf
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Marc: |
LEADER 02831naa a2200361 a 4500 001 2105692 005 2020-01-07 008 2018 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1186/s12885-018-4077-3$2DOI 100 1 $aPEREIRA, C. M. 245 $aIn vitro and in silico validation of CA3 and FHL1 downregulation in oral cancer.$h[electronic resource] 260 $c2018 500 $aArticle number: 193. 520 $aAbstract. Background: Aberrant methylation is a frequent event in oral cancer. Methods: In order to better characterize these alterations, a search for genes downregulated by aberrant methylation in oral squamous cell carcinoma (OSCC) was conducted through the mining of ORESTES dataset. Findings were further validated in OSCC cell lines and patients? samples and confirmed using TCGA data. Differentially expressed genes were identified in ORESTES libraries and validated in vitro using RT-PCR in HNSCC cell-lines and OSCC tumor samples. Further confirmation of these results was performed using mRNA expression and methylation data from The Cancer Genome Atlas (TCGA) data. Results: From the set of genes selected for validation, CA3 and FHL1 were downregulated in 60% (12/20) and 75% (15/20) of OSCC samples, respectively, and in HNSCC cell lines. The treatment of cell lines JHU-13 and FaDu with the demethylating agent 5'-aza-dC was efficient in restoring CA3 and FHL1 expression. TCGA expression and methylation data on OSCC confirms the downregulation of these genes in OSCC samples and also suggests that expression of CA3 and FHL1 is probably regulated by methylation. The downregulation of CA3 and FHL1 observed in silico was validated in HNSCC cell lines and OSCC samples, showing the feasibility of integrating different datasets to select differentially expressed genes in silico. Conclusions: These results showed that the downregulation of CA3 and FHL1 data observed in the ORESTES libraries was validated in HNSCC cell lines and OSCC samples and in a large cohort of samples from the TCGA database. Moreover, it suggests that expression of CA3 and FHL1 could probably be regulated by methylation having an important role the oral carcinogenesis. 650 $aGene expression 650 $aMethylation 650 $aMetilação 653 $aCarcinogênese 653 $aExpressão gênica 653 $aOpen reading expressed sequence tags 653 $aOral squamous cell carcinoma 653 $aORESTES 653 $aValidação in silico 653 $aValidação in vitro 700 1 $aCARVALHO, A. C. de 700 1 $aSILVA, F. R. da 700 1 $aMELENDEZ, M. E. 700 1 $aLESSA, R. C. 700 1 $aANDRADE, V. C. C. 700 1 $aKOWALSKI, L. P. 700 1 $aVETTORE, A. L. 700 1 $aCARVALHO, A. L. 773 $tBMC Cancer$gv. 18, p. 1-10, 2018.
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